The sequencing platform of Lille GO@L
GO@L GEnOmique-at-Lille) is the genomics platform of the PLBS unit (UAR2014-US41). It provides a wide range of solutions and expertise in the field of high-throughput genomics. The technical approaches used are mainly based on high-throughput sequencing. The platform is the result of the merger of two specific and long-established entities, TAG (Transcriptomic and Applied Genomic) and GFS (Functional and Structural Genomics), and over the years has developed recognised expertise in microbiology and human genomics.
GO@L offers RNA quantification approaches (mRNA, small RNA, lncRNA, isoforms, single-cell, spatial) in a targeted manner or at the genome level for differential expression analyses, approaches for the identification and quantification of DNA anomalies (target-Seq, WES, WGS), for the regulation of gene expression (DNAse-Seq, methylation) GO@L has also developed a specialisation in microbial genomics with whole genome sequencing applications for bacteria and viruses, classical and dedicated RNA-seq applications such as dual RNA-seq, differential RNA-seq, full-length RNA-seq (Long Reads) and a complete pipeline of targeted metagenomic studies.
The GO@L team is composed of half molecular biologists specialised in high throughput and half bioanalysts and bioinformaticians who are experts in the analysis of the data generated and the development of dedicated solutions.
Expertise
- Sequencing and re-sequencing of whole genomes (human, bacteria, parasites, viruses),
- Resequencing of human exomes of various qualities (FFPE, PBMC),
- Targeted sequencing (amplicons, captures),
- RNA-Seq, 3'RNA-Seq and sRNA-Seq (human, mouse, bacteria),
- RNA-seq full-length (Long Reads)
- Identification of transcription sites (TSS) by RNA-Seq,
- scRNA-Seq (10X genomics, tapestri),
- spatial transcriptomics (10X visium),
- 16S targeted metagenomics,
- qRT-PCR
Equipment
- Nova-Seq 6000 Illumina,
- MiSeq Illumina,
- MinION, PromethION2 Oxford Nanopore Technologies.
- Chromium 10X Genomics,
- Tapestri
Bioinformatics
Bioinformatics analysis: GO@L has developed, for collaborative projects, analysis expertise associated with the applications proposed by the platform. For genomic characterisation: alignment on reference, assembly, detection and annotation of variants, Mendelian transmission study, clonality study, CNV analysis. For transcriptomics: DGE, RNA splicing analysis, gene prediction/discovery. For targeted metagenomics: taxonomic assignment, diversity assessment, dysbiosis measurement.
Main Achievements
Most of GO@L’s activity is carried out in collaboration with a research laboratories. GO@L has thus been a partner in many large-scale projects financed by major funding agencies (ANR, ERC, H2020, etc.). Within the collaboration, the research laboratory usually leads the project and the GO@L platform supports the project by providing its expertise and know-how and as such is associated to publications. However, from time to time, our platform acts as a project leader and carries out the work itself, usually on the development and application of methods or protocols (D’halluin et al., 2025; Amman et al, 2018; Siegwald et al., 2017; Hot et al., 2011) or on the development or improvement of bioinformatics solutions(CuReSim-LoRM (Mesloub et al., 2023), V-ASAP (Maurier et al, 2019), DiNAMO (Saad et al., 2018), cghRA (Mareschal et al. 2017), MICRA (Caboche et al, 2017), MPAgenomics (Grimonprez et al. 2014), vidjil (Giraud et al., 2014), (WACA (Leprêtre et al., 2010). Several reviews have also been published by the platform on its areas of expertise and their perspectives (Audebert et al., 2018, Caboche et al., 2014(a); Caboche et al., 2014(b); Audedert et al., 2014; Jardin et al., 2013; ).
Last update February 2025
Certification / Quality Assurance
Labels
IBISA
Platform Managment
Martin Figeac
David Hot
PLBS UAR 2014-US
Pôle Recherche
1 place de Verdun
59045 Lille cedex
Contacts
